The basophil degranulation test (also known as basophil activation test [BAT] or CAST test) reproduces the allergic immediate type reaction in the test tube after addition of the suspected allergen to basophil granulocytes enriched from the patient blood. In case of allergic sensitisation, released leukotrines are detected.The test thus resembles the reaction in the prick test but with the advantage of being independent of antihistamine ingestion, dermatographic urticaria and other factors that influence skin tests. There is also no risk of triggering anaphylaxis with an in vitro test.
Unlike IgE detection in the CAP test, the BDT also detects basophil-bound allergen-specific IgE antibodies and is therefore very sensitive.
As a classic ‘in vitro provocation test’ the BDT is also suitable for the detection of immediate type hypersensitivity reactions not mediated by IgE (pseudoallergies/idiosyncrasies to some medications, work-place and environmental allergens, food additives and dyes).
Regarding sensitivity and specificity, the BDT has proven to be clearly superior in our laboratory compared to other in vitro provocation tests such as the histamine release test or the CD63 test.
Detection of IgE-mediated type I sensitisations:
In the list you will find the validated allergens available in the laboratory.
For allergens not listed an allergen sample (for medi-cations tablet or ampoule and for other materials about 2 g or 0.5 ml of substance) must be sent to-gether with the blood sample.
2 ml heparin blood or EDTA for each allergen
Sample receipt within 24 hrs has to be ensured. The sample should be stored and transported at room temperature. Within the Berlin city area, we offer a courier service (+49 (0)30 7701- 250). For collections beyond Berlin, please contact our complimentary courier service (+49 (0)30 77001- 450).